Fig. 4: ICD induced phagocytosis of IR-treated GSCs, DCs phenotypic maturation, cytokine production.
From: Bip inhibition in glioma stem cells promotes radiation-induced immunogenic cell death
DCs were generated from PBMCs of three healthy doors after culture of adherent monocytes with rhGM-CSF and rhIL-4 for 7 days. GSCs transfected with or without siRNA-Bip were irradiated with 10 Gy and incubated for 24 h, then were added to human DCs at ratio 1:1 and incubated for additional 24 h, then the characteristics of DCs were determined. a The percentage of DCs positive for CFSE dye after co-culture with GSCs for far red dye was assessed by flow cytometry, and the percentage of double positive cells (DCs/GSCs) was calculated. b Specific markers expression of DCs maturation was evaluated by flow cytometry using monoclonal antibodies. c The levels of IL-10 and IL-12 was examined from cell culture supernatants by elisa. #P < 0.05, ##P < 0.01.
