Fig. 5: USP29 expression enhances stemness of lung adenocarcinoma cells and in vivo tumorigenesis in xenograft mouse model. | Cell Death & Disease

Fig. 5: USP29 expression enhances stemness of lung adenocarcinoma cells and in vivo tumorigenesis in xenograft mouse model.

From: USP29 enhances chemotherapy-induced stemness in non-small cell lung cancer via stabilizing Snail1 in response to oxidative stress

Fig. 5

a, b Side population was identified in H1299 and H1975 cells stably transfected with control or USP29-expressing vectors with the presence and absence of verapamil (200 μM). Scatter plots show representative flow cytometric analysis from three independent experiments for H1299 and H1975 as indicated, with solid boxes indicating side population cells. c, d The relative quantification of side population cells from (a, b), respectively (n = 3). e The capabilities of H1299-pCDH and H1299-pCDH-USP29 stable cell lines to form tumor xenograft in athymic nude mice were compared in parallel, with the image showing resected xenograft tumors. f The volumes of tumor xenograft formed by two cell lines during the experimental period were measured and plotted. g Weights of tumor xenografts resected were measured and plotted. h Tumor xenograft tissues were processed for Western blotting analysis to probe for USP29 and Snail1 levels. Tubulin blot shows equal loading. i Quantification data from (h). All error bars represent the standard error of the mean (SEM), with *p value < 0.05 and **p value < 0.01.

Back to article page