Fig. 4: RNF115 interacts with STX17 and enhances its stability.

a–c HEK293T cells were cotransfected with indicated plasmids for 24 h, then the cell lysates were subjected to GFP-TRAP beads. The interactions were analyzed by western blotting with anti-RNF115, anti-GFP, or anti-MYC antibodies, respevtively. GAPDH was used as the loading control. d HEK293T cells were transfected with GFP-STX17 for 24 h, the cell lysates were subjected to IP using either an anti-GFP antibody or an IgG isotype control. The interaction was analyzed by western blotting with anti-RNF115 antibodies. GAPDH was used as the loading control. e Hela cells were cotransfected with GFP-STX17 and shcontrol or shRNF115 for 24 h, the levels of GFP-STX17 were analyzed by western blotting. f Cells were treated as in e, the fluorescence of GFP-STX17 was analyzed by confocal microscope. Scale bar, 25 μm. g HEK293T cells were cotransfected with indicated plasmids for 24 h, with or without EBSS for another 2 h, then the levels of GFP-STX17 were analyzed by western blotting.