Fig. 2: AML Blasts express functional P2X7RA and P2X7RB. | Cell Death & Disease

Fig. 2: AML Blasts express functional P2X7RA and P2X7RB.

From: Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression

Fig. 2

a Extracellular ATP (pM) was measured in the culture supernatants as described in materials subjects and methods. AML blasts were plated and treated for 6 h with vehicle (PBS, black) and DNR (200 nM, red). mRNA expression of b P2X7RA and c P2X7RB in three representative AML patient blasts treated with vehicle (PBS, black) and DNR (200 nM, red). d Representative traces showing an increase of intracellular calcium following stimulation with 500 µM BzATP of HIGH-P2X7RA AML patient blasts (gray) and HIGH-P2X7RB AML patient blasts (blue). e Representative traces showing ethidium bromide uptake following stimulation with 500 µM BzATP of HIGH-P2X7RA AML patient blasts (gray) and HIGH-P2X7RB AML patient blasts (blue). Data are represented as mean ± SEM. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.

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