Fig. 1: miR-200b/c-3p directly target to RAC1 3′UTR. | Cell Death & Disease

Fig. 1: miR-200b/c-3p directly target to RAC1 3′UTR.

From: MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling

Fig. 1

a Venn diagram depicting predicted miR-200b/c-3p target gene numbers from two algorithms. b Top pathways enriched for putative miR-200b/c-3p target genes with potential binding sites in 3′UTRs. DAVID was used for gene ontology enrichment analysis. c Putative miR-200b/c-3p binding site in RAC1 3′UTR is conserved among vertebrates. miRNA-mRNA hybridization structures and folding energy was predicted by RNAhybrid. d Schematic representation of luciferase reporter construct with full length RAC1 3′UTR. e Luciferase reporter assay confirmed miR-200b/c-3p repressed RAC1 by direct binding to a conserved binding site in 3′UTR. Renilla luciferase activity was normalized to firefly luciferase activity. Relative luciferase activities were ratios of Renilla/Firefly luciferase normalized to negative control for each reporter construct. A reporter with reverse complement sequence of miR-200b/c-3p (200luc) was served as positive control (n = 4). Data presented as mean ± SD. **P < 0.01, ***P < 0.001 versus negative control, one-way ANOVA.

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