Fig. 2: miR-200b/c-3p repress RAC1 signaling in keratinocytes. | Cell Death & Disease

Fig. 2: miR-200b/c-3p repress RAC1 signaling in keratinocytes.

From: MicroRNA-200b/c-3p regulate epithelial plasticity and inhibit cutaneous wound healing by modulating TGF-β-mediated RAC1 signaling

Fig. 2

a Mice dorsal skin was wounded and wound edge samples were collected at the indicated time points post wounding for western blotting. Representative western blot and quantitative data showed RAC1 protein level was elevated in mice skin wounds (n = 6). b RT-qPCR analysis of wound edge samples collected as in (a) showed miR-200b/c-3p expression was decreased in mice skin wounds (n = 6). c Confluent HaCaT cells were scratch wounded and samples were collected at the indicated time points post wounding for western blotting. Representative western blot and quantitative data showed RAC1 protein level was elevated in HaCaT cell scratch wounds (n = 3). d RT-qPCR analysis of samples collected as in (c) showed miR-200b/c-3p expression was decreased in HaCaT cell scratch wounds (n = 3). e Western blot analysis of RAC1 in HaCaT cells transfected with RNA duplexes as indicated. Representative western blot and quantitative data showed RAC1 protein levels were significantly reduced by miR-200b/c-3p duplexes or siRNA (n = 3). dpw, days post-wounding; hpw, hours post-wounding. Data presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 versus control, one-way ANOVA.

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