Fig. 4: miR-18a mediates chondrocyte hypertrophy via inhibiting TGF-β signaling. | Cell Death & Disease

Fig. 4: miR-18a mediates chondrocyte hypertrophy via inhibiting TGF-β signaling.

From: Targeting miR-18a sensitizes chondrocytes to anticytokine therapy to prevent osteoarthritis progression

Fig. 4

a qRT-PCR to determine the expression of IL-1β, miR-18a, and SERPINE1 in injured cartilage and corresponding paired unaffected cartilage of OA patients. b Pearson’s correlation coefficient between IL-1β, IKBα (NF-κB signaling downstream gene), miR-18a, RUNX2, SERPINE1, AXIN2, and ID1 in injured lesions of OA patients was performed, and the heat map shows r values. c Correlation between p-SMAD2/3 and expression of miR-18a in OA lesions was assessed by IHC, qRT-PCR, and chi-square test. d IHC to determine the level of phosphorylation of SMAD2/3 in unaffected and injured articular cartilage. Scale bar: 50 μm. e Effects of restored expression of TGFβ1, SMAD2, or SMAD3 in miR-18a-overexpressing cells on expression of hypertrophy-related genes. f qRT-PCR exhibits the expression of hypertrophy-related genes in AC and SW1353 cells expressing inhibitor of miR-18a with or without LY2109761. Data in f are presented as mean ± SD. *P < 0.05.

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