Fig. 5: IL-1β-mediated hypertrophy depends on miR-18a-induced suppression of TGF-β signaling. | Cell Death & Disease

Fig. 5: IL-1β-mediated hypertrophy depends on miR-18a-induced suppression of TGF-β signaling.

From: Targeting miR-18a sensitizes chondrocytes to anticytokine therapy to prevent osteoarthritis progression

Fig. 5

a Effects of IL-1β on activation of TGF-β signaling in AC and SW1353 cells expressing NC or miR-18a inhibitor were assessed. b Expression of hypertrophy-related genes was analyzed in NC- or miR-18a inhibitor-expressed cells in response to IL-1β. c TGF-β signaling downstream genes expression was analyzed in IL-1β-suppressed cells with or without miR-18a re-expression. d Effects of LY2109761 on chondrocyte hypertrophy in cells with IL-1β treatment and miR-18a inhibitor expression were determined. e qRT-PCR shows the expression of hypertrophy-related genes when TGFβ1, SMAD2, or SMAD3 was restored expressed in cells with IL-1β stimulation. f Activity of NF-κB and TGF-β signaling, and expression of RUNX2 were determined in rat model of OA in Fig. 1a. Scale bar: 50 μm. g, h Activity of TGF-β signaling and the expression of hypertrophic genes in chondrocytes were assessed in cells in response to TNFα with or without inhibition of miR-18a. Data in a–c are presented as mean ± SD. *P < 0.05.

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