Fig. 6: Downregulation of TRAF3 inhibited the activation of MAPKs and NF-κB signaling pathway in SAH primary neuronal cells. | Cell Death & Disease

Fig. 6: Downregulation of TRAF3 inhibited the activation of MAPKs and NF-κB signaling pathway in SAH primary neuronal cells.

From: TRAF3 mediates neuronal apoptosis in early brain injury following subarachnoid hemorrhage via targeting TAK1-dependent MAPKs and NF-κB pathways

Fig. 6

A Western blotting of MAPKs at 24 h after SAH. The P38, P44/42, and JNK expression levels were unaltered. the expression of p-P38, p-P44/42, and p-JNK increased in the SAH group compared with the control group. Quantitative analyses showed that the downregulation of TRAF3 reduced the activation of p-P38 (B), p-P44/42 (C), and p-JNK (D). E Western blotting of the NF-κB signal pathway in 24 h after SAH. The protein expression levels of NF-κB nuclear subunits p-P65 (F) and P65 (G) were increased in SAH and SAH + control siRNA groups and downregulated in the SAH + TRAF3 siRNA group. Levels IκBα was decreased after SAH, TRAF3 siRNA treatment increased the protein level of IκBα (H). N = 6 wells per group. *p < 0.05 versus the control group, #p < 0.05 versus the SAH group.

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