Fig. 7: TRAF3–TAK1 interaction and phosphorylation of TAK1 are required for TRAF3-dependent neuronal apoptosis in SAH. | Cell Death & Disease

Fig. 7: TRAF3–TAK1 interaction and phosphorylation of TAK1 are required for TRAF3-dependent neuronal apoptosis in SAH.

From: TRAF3 mediates neuronal apoptosis in early brain injury following subarachnoid hemorrhage via targeting TAK1-dependent MAPKs and NF-κB pathways

Fig. 7

Western blot analysis revealed that TRAF3 siRNA reduced the phosphorylation of TAK1 after SAH both in vivo (A, B) and in vitro (C, D). N = 6 mice or wells per group. *p < 0.05 versus the control group, #p < 0.05 versus the SAH group. E Co-immunoprecipitation showed that the expression of TRAF3 could interact with TAK1 in SAH primary neural cells. F, G Western blotting analysis showed that AdTRAF3-M failed to increase the expression of p-TAK1 in primary neural cells while AdTRAF3 succeeded. H–N Western blotting analysis showed that the activation of MAPKs and NF-κB signaling was potentiated by AdTRAF3, but abrogated by AdTRAF3-M. N = 6 wells per group. *p < 0.05 versus the control group.

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