Fig. 6: ATG12 knockdown potentiated the radiosensitivity of CRC cells by inhibiting autophagy. | Cell Death & Disease

Fig. 6: ATG12 knockdown potentiated the radiosensitivity of CRC cells by inhibiting autophagy.

From: Long non-coding RNA HOTAIR knockdown enhances radiosensitivity through regulating microRNA-93/ATG12 axis in colorectal cancer

Fig. 6

a, b Transfection efficiency analysis of siATG12 in SW480 and HCT116 cells through western blot assay at 48 h after transfection. c–h The effects of ATG12 knockdown on cell radiosensitivity, viability, and apoptosis were measured by radiation clonogenic survival assay (c, d), CCK-8 assay (e, f), and flow cytometry analysis (g, h). i The effects of ATG12 silencing alone or in combination with IR stimulation on protein expression of LC3 I, LC3 II, p62, cleaved caspase 3, Bax, and Bcl-2 were measured by western blot assay. *P < 0.05.

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