Fig. 2: Ferroptosis was induced in colitis in mice.

a Representative images from H&E staining of colonic sections from DSS-treated mice and vehicle mice (Scale: 100 μm). b Immunohistochemical staining for MPO of colonic sections (Scale: 100 μm). c PI-positive cells were detected by flow cytometry to analysis necrotic cell death in colonic epithelial tissues of mice. d Iron levels of colonic epithelial tissues of mice were determined by Iron Assay Kit. e MDA levels were detected in colonic epithelial tissues of mice according to MDA Assay Kit. f Transmission electron micrographs of colonic epithelial cells from experimental colitis and vehicle mice (Scale: 500 nm). Blue arrows indicate shrunken mitochondria and red arrows indicate reduction of mitochondria crista. g Western blotting analysis of FTL and FTH in colonic epithelial tissues. β-actin was used as the loading control. h Double immunofluorescent staining for FTH and CK 18 were performed in the colonic sections of mice. Nuclei was stained with DAPI in blue. Localization of FTH was visualized in green and CK 18 was stained in red, the merging positive signals were visualized in yellow (Scale: 50 μm). i Relative mRNA level of PTGS2 and GPX4 in colonic epithelial tissues from mice with or without DSS administration were measured by real-time PCR. Statistical analyses were performed with Student’s t tests.