Fig. 5: ZNF750 may act as a tumor suppressor via DANCR/miR-4707-3p/FOXC2 axis that works in a ceRNA manner in ESCC.

a, b DANCR silence led to a decrease of FOXC2 protein level whereas augmentation of DANCR caused an increase in vitro (a), and the inhibition of FOXC2 protein was partially abolished by DANCR-wt overexpression in KYSE150-miR-4707-3p mimics cells (b). β-Actin was used as control. Statistical analysis was performed with a two-sided t test and one-way ANOVA. c Dual luciferase assay showed that the inhibitory effect of miR-4707-3 on FOXC2-3’-UTR reporter was partially abolished by DANCR-wt overexpression. P values were obtained using ANOVA. d–f Immunohistochemistry (d, e) and qRT-PCR (f) showed the expression of FOXC2 in DANCR knockdown or overexpression xenograft mouse samples. GAPDH was used as loading control. Statistical analysis was performed with a two-sided t test. g–j Western blot (g, h) and qRT-PCR (i, j) showed the expression of DANCR, miR-4707-3p, FOXC2 in ZNF750 knockdown and overexpression cells. β-Actin, GAPDH and U6 were used as loading control respectively. Statistical analysis was performed with a two-sided t test. (k–m) Immunohistochemistry (k, l) and qRT-PCR (m) were used to detect the expression of DANCR, FOXC2 in ZNF750 overexpression xenograft mouse samples. GAPDH was used as loading control. Statistical analysis was performed with a two-sided t test. *p < 0.05, **p < 0.01, *** p < 0.001.