Fig. 1: TPL treatment inhibited mitochondrial OXPHOS and reduced ROS level. | Cell Death & Disease

Fig. 1: TPL treatment inhibited mitochondrial OXPHOS and reduced ROS level.

From: The heterocyclic compound Tempol inhibits the growth of cancer cells by interfering with glutamine metabolism

Fig. 1

a MTT assay of SKOV3 cells treated with 1 mM TPL and control. b Effect of TPL in different concentrations on intracellular ROS production from drug treatment for 24 h by DCFH-DA-dependent measurements using flow cytometry analysis. c, d SKOV3 cell were treated with control (black) or 0.8 mM TPL (purple) for indicted time before measurement of OCR detected by Seahorse XFe96 Extracellular Flow analyzer in real time. Arrow is the time of inhibitors adding sequential. Oligomycin (1 μM), FCCP (0.5 μM), and rotenone-antimycin A (0.5 μM). The bracketed concentrations of drugs are the final concentrations. Histogram of OCR rate was shown d. e The baseline OCR of SKOV3 cells after treating with indicated concentration of TPL for 12 h. For all experiments, values are mean ± SD carried out in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001; no sign means no significant difference; Student’s t-test.

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