Fig. 5: TPL inhibited the IDH1 and IDH2 enzyme activity. | Cell Death & Disease

Fig. 5: TPL inhibited the IDH1 and IDH2 enzyme activity.

From: The heterocyclic compound Tempol inhibits the growth of cancer cells by interfering with glutamine metabolism

Fig. 5

a The effects of TPL on the phosphorylation of IDH1 and IDH2 in SKOV3 cells were analyzed by a Phos-tag assay. Immunoblots for total IDH1 and IDH2 were also performed on normal SDS–PAGE gels. b, c Enzymatic activity of both IHD1 and IDH2 treated with control and TPL (n = 3). For all experiments, values are mean ± SD carried out in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001; no sign means no significant difference; Student’s t-test.

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