Fig. 1: Identification of extracellular vesicles (EVs) secreted by hESC-CVPCs.

a Transmission electron microscope revealed that hESC-CVPCs contain multivesicular bodies (MVBs). b Extracellular vesicles isolated from hESC-CVPC-conditioned medium showed typical cup-shaped shapes at diameter ~30–150 nm. c, d The nanoparticle tracking analysis of the mode size c and the particle concentrations for the EVs secreted from hCVPCs with (EV-H) and without hypoxia treatment (EV-N) in the culture supernatant of hCVPCs (d). n = 5. e Western blot analysis of the EV markers, Alix, TSG101,CD63, CD9, and CD81. Scale bar, 100 nm.