Fig. 2: Elevation of ROR2 suppressed migration and invasion of PCa cells.

(a) ROR2 protein level in nonmalignant PZ-HPV-7, RWPE-1 cells prostate epithelial cells, and commonly used PCa cell lines (CA-HPV-10, LNCaP, LNCAP C4-2B, DU-145, PC-3) was examined by western blot. GAPDH was used as loading control. Cell migration and invasion of control PC-3 cells and PC-3 cells overexpressing ROR2 (pCMV-ROR2-1 and pCMV-ROR2-2) (b), control DU-145 cells and DU-145 cells overexpressing ROR2 (c), control C4-2B cells and C4-2B cells overexpressing ROR2 (d), as well as control RWPE-1 cells and RWPE-1 cells with ROR2 shRNA knockdown (e) were determined by the transwell assay. Asterisks *, **, and *** represent statistically significant p < 0.05, p < 0.01, and p < 0.001, respectively, between the two groups being compared. Cell migration of PC-3 (f) or DU-145 (g) cells with or without ROR2 overexpression was also examined by wound healing assay. Images were obtained by live imaging microscope (Leica AF 6000 LX, Leica, Wetzlar, Germany). The Effect of the overexpression of ROR2 on proliferation of PC-3 (h), DU-145 (i), and C4-2B (j) PCa cells as well as effect of shRNA knockdown of ROR2 on RWPE-1 (k) cells for 24, 48, 72, and 96 h was determined by the proliferation assay. Asterisks * and ** represent statistically significant p < 0.05 and p < 0.01, respectively, between the two groups being compared.