Fig. 4: Tfap2c inhibits c-Myc-dependent apoptosis.

a Microscopy of reprogramming cells at post-infection day 4, and the number of OSKM and OSKMT reprogramming cells at different time points was recorded. Scale bar, 100 μm. b Immunostaining of Ki67 in OSKM and OSKMT groups at day 4. Scale bar, 50 μm. c Flow-cytometer analysis of cell apoptosis after infected with OSK, OSKT, OSKM, and OSKMT on day 4. d The percent of Annexin V⁺ cells in OSK, OSKT, OSKM, and OSKMT group on day 4. e Immunoblotting of Cleaved caspase-3 (CC3) and Cleaved PARP1 (CPARP1) in MEFs after infected with OSK, OSKT, OSKM, and OSKMT on day 4. f Immunoblotting of CC3 and CPARP1 in MEFs after infected with OSK, OSKT, OSKM, and OSKMT on day 8. g Immunoblotting of CC3 and CPARP1 in MEFs after infected with OSKM and OSKMT (day 4) with or without PAC-1 (1.25 μM) treatment. h Number of Oct4-GFP⁺ colonies (day 12) in reprogramming cells treated with or without PAC-1. Significance in panels d was determined by one-way ANOVA with Dunnett’s test. Significance in panels a and h was determined by two-way AVOVA with Sidak’s multiple comparisons test. Significance summary: P > 0.05 (ns); *P ≤ 0.05; ***P ≤ 0.001. All data are presented as mean ± S.D., N = 3.