Fig. 5: Acetyl-CoA regulates acetylation of H4 histones and CSC-associated gene expressions. | Cell Death & Disease

Fig. 5: Acetyl-CoA regulates acetylation of H4 histones and CSC-associated gene expressions.

From: Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation

Fig. 5

a Protein levels of acetylated-lysine histone H3 and H4 were determined by immunoblotting in cell lysates from Hs578T and BT549 spheres treated with or without KU60019 (10 μM) under hypoxia. β-Actin is the loading control. b Immunofluorescence staining to check acetylated-H4ac levels in Hs578T and BT549 mammospheres treated with or without KU60019 (scale bar, 100 µm) under hypoxia. c–e The status of H4 acetylation was determined in hypoxic Hs578T and/or BT549 mammospheres suffered to different treatments. c Spheres treated with KU60019 (10 μM); d those treated with 2-DG (5 mM), UK5099 (15 μM), and BMS303141 (20 μM), respectively; e those treated with acetate (10 mM). Total H4 is the loading control.

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