Fig. 1: Zinc treatment affects ferroptosis susceptibility.
From: Zinc transporter ZIP7 is a novel determinant of ferroptosis
A MDA-MB-231 cells were treated with DMSO, 5 μM TPEN (zinc chelator), 100 μM DFO (iron chelator) together with 10 μM erastin for 21 h. B HT-1080 cells were treated with DMSO, 5 μM TPEN (zinc chelator), 100 μM DFO (iron chelator) together with 10 μM erastin for 24 h. The data were represented as mean ± SD (n = 6; ****p < 0.0001; two-way ANOVA) (C) MDA-MB-231 cells were treated with 4 μM erastin alone or combined with 100 μM ZnCl2 or 25 μM Ferric Citrate for 24 h. The data were represented as mean ± SD (n = 3; ****p < 0.0001; two-way ANOVA). D Zinc addition mitigated the ferroptosis protective effects of an iron chelator. MDA-MB-231 cells were treated with 5 μM DFO, 25 μM ferric citrate, 100 or 200 μM ZnCl2 10 μM erastin for 24 h. E HT-1080 cells were treated with 5 μM DFO, 25 μM ferric citrate, 100 or 200 μM ZnCl2, and 10 μM erastin for 24 hours. The data were represented as mean ± SD (n = 6; ***p = 0.0001; ****p < 0.0001; two-way ANOVA).
