Fig. 4: Cep70-knockout mice showed germ-cell apoptosis and abnormal spermiogenesis.
From: Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis
A MVH immunofluorescence staining of testes sections of the WT and Cep70−/− mice, demonstrating a significant decrease of germ cells in the seminiferous tubules of Cep70−/− mice. Scale bar: 10 μm (left), 20 μm (right). B Quantification of germ cells in each seminiferous tubule of the WT (n = 32) and Cep70−/− mice (n = 40) at 10-weeks of age. Data are presented as the mean ± SD, p < 0.001 (***). C TUNEL immunofluorescence staining of testes sections of the WT and Cep70−/− mice. The TUNEL-positive signal (green) level was significantly higher in Cep70−/− mice. Scale bar: 500 μm (up), 50 μm (down). D PAS-hematoxylin staining of seminiferous tubules from the WT and Cep70−/− mice, indicating spermatogenic arrest at stages VII–VIII in the Cep70−/− mice. Scale bar: 20 μm.
