Fig. 4: Mut-H USCs had elevated UPRmt and reduced GSK3B and WNT7B.
From: ATF5, a putative therapeutic target for the mitochondrial DNA 3243A > G mutation-related disease
A The mRNA levels of UPRmt target genes (ATF5, HSP70, HSP60 and LONP1) were elevated in Mut-H USCs. Total RNAs were extracted from Ctr, Mut-L and Mut-H USCs, and expression of UPRmt target genes were quantified through real-time quantitative PCR (RT-qPCR). B–G The protein levels of UPRmt target genes (ATF5, HSP70, HSP60 and LONP1) were elevated in mutant-high USCs. Total protein lysates were extracted from USCs and protein levels were analyzed by western blot. β-actin served as internal loading control. C, E, G were quantification plot for B, D, F, respectively. H mRNA levels of WNT7B were reduced in Mut-H USCs. I Protein levels of p-GSK3β were decreased in Mut-H USCs. GADPH serves as internal control. J Quantification plot for I. *P < 0.05, **P < 0.01, ***P < 0.001.
