Fig. 5: Combination of 17-AAG and DDP can efficiently inhibit the growth of ovarian cancer cells in vitro. | Cell Death & Disease

Fig. 5: Combination of 17-AAG and DDP can efficiently inhibit the growth of ovarian cancer cells in vitro.

From: Lamin-A interacting protein Hsp90 is required for DNA damage repair and chemoresistance of ovarian cancer cells

Fig. 5

A HO-8910 cells or stable lamin-A knockdown cells were treated with DDP (2.5 and 5 μM) or 17-AAG (0.5 μM) for 96 h, and cell viability was analyzed by CCK-8 assay. B Clonogenic assay: representative images of plates and colony numbers of different groups. Colonies containing more than 50 cells were counted. C and D Analysis and quantifications of IC50 value in HO-8910 cells (or stable lamin-A knockdown cells) treated with different concentrations of DDP (or combined with 17-AAG) for 48 h C and 72 h D. E Representative images of γ-H2AX foci and quantitative results of γ-H2AX positive cells. Scale bars, 50 μm. All the experiments were repeated three times. All the error bars indicated means ± SD. Statistical significance was concluded at *p < 0.05; **p < 0.01; ***p < 0.001.

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