Fig. 6: USP28 interacting with FOXM1 and stabilising FOXM1 expression via deubiquitination. | Cell Death & Disease

Fig. 6: USP28 interacting with FOXM1 and stabilising FOXM1 expression via deubiquitination.

From: USP28 facilitates pancreatic cancer progression through activation of Wnt/β-catenin pathway via stabilising FOXM1

Fig. 6

A–D The interaction between USP28 and FOXM1 was confirmed by co-immunoprecipitation (IP) in SW1990 cells and BxPC-3 cells. E, F Co-localisation studies of PC cells using anti-USP28 antibody (1:100, green) and anti-FOXM1 antibody (1:100, red), followed by DAPI nuclear counterstaining (blue). The merged images of USP28 (green) and FOXM1 (red) with DAPI (blue) are also shown. Scale bar, 50 μm. G, H Representative (right) and quantitative (left) results of FOXM1 protein level in USP28-silencing cells. The cells were treated with cycloheximide (CHX, 100 mg/ml) for indicated time points were subjected to western blot analysis. **P < 0.01. I J PC cells transduced with p-USP28 (I) or shUSP28 (J) were treated with 10 μM MG132. Cells were collected at 6 h and immunoblotted with the antibodies indicated. K, L Lysates from PC cells transduced with p-USP28 (K) or shUSP28 (L) were immunoprecipitated with the anti-Ub and immunoblotted with the anti-FOXM1. Cells were treated with MG132 for 6 h before collection.

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