Fig. 2: Increased PKLR and decreased ZBTB10 are associated with NED of PCa after ADT. | Cell Death & Disease

Fig. 2: Increased PKLR and decreased ZBTB10 are associated with NED of PCa after ADT.

From: Pyruvate kinase L/R links metabolism dysfunction to neuroendocrine differentiation of prostate cancer by ZBTB10 deficiency

Fig. 2

A Mean expression of ZBTB10 and PKLR in LNCaP cells from the GDS3358 database during 11 months of ADT. * vs. the control. **p < 0.01, ***p < 0.001; by a one-way ANOVA. B PKLR and ZBTB10 expression in patient samples of the GSE48403 dataset showed an increase in PKLR and a decrease in ZBTB10 expression after ADT. *p < 0.05, **p < 0.01; by a one-way ANOVA. C Protein levels of ZBTB10 in various PCa cells. D PKLR and ZBTB10 mRNA levels in C4-2 cells during 5 months of 20 μM MDV3100/enzalutamide treatment compared to parental C4-2 cells, by RT-qPCR analysis. * vs. parental C4-2; by a one-way ANOVA. E RT-qPCR showing PKLR, ZBTB10, NE marker, stem cell marker, and androgen-responsive gene mRNA levels in C4-2 cells following treatment with charcoal-stripped serum (CSS)-containing medium for 48 h, and further treatment with 10 nM dihydrotestosterone (DHT) for 24 h. * vs. -CSS; # vs. +CSS; by a two-way ANOVA. Quantification of mRNA is presented as the mean ± SEM from three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001. F Immunoblots showing PKLR, ENO2, CHGA, ZBTB10, NKX3-1, and KLK3 protein levels in LNCaP and C4-2 cells following treatment with CSS-containing medium for 5 and 10 days and 10 nM DHT for 24 h. G, H Representative images of IHC staining and intensity analysis (by an H-score analysis) of PKLR (G) and ZBTB10 (H) in PCa tissue sections from the same patients before and after ADT. n = 17 samples from Taipei Medical University-Wan Fang Hospital. Scale bars, 100 µm. Statistical analysis by two-tailed Student’s t-test. ****p < 0.0001.

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