Fig. 4: MIF K78 acetylation impaired neuronal death after ischemia.
From: Macrophage migration inhibitory factor (MIF) acetylation protects neurons from ischemic injury
A Diagram of the experimental procedure. B MIF K78 acetylation attenuated ischemic neuronal death. Mice were injected with AAV-shMIF-GFP together with either AAV-MIF-2A-mCherry or AAV-K78Q-2A-mCherry and ischemia was induced. Infarct volume was analyzed by TTC staining in brain sections. C Quantification of the lesion volume is shown in (B). Data shown were independent points and mean; one-way ANOVA; n = 6; ***p < 0.001. D–F Neurological deficits were evaluated by the mNSS test (D), foot fault assays (E), and adhesive-removal test (F). Quantification analysis of behavioral tests. Data shown were mean ± SEM; two-way ANOVA; n = 6; ***p < 0.001. G Reduced DNA fragmentation in neurons expressing MIF K78Q after ischemic stroke. The brain sections infected with indicated AAV were stained with an anti-γH2AX antibody. Arrows indicate AAV-infected neurons. H Quantification of the γH2AX-positive cells were shown in (G). Data shown were independent points and mean; one-way ANOVA; n = 6; ***p < 0.001.
