Fig. 6: Reduced brain injury in MIF K78Q knock-in mice after ischemia.

A Attenuated infarct volume in MIF K78Q mice after ischemic stroke. Ischemia was induced in WT and MIF K78Q mice and infarct volume was analyzed by TTC staining in brain sections. B Quantification analysis of lesion volume shown in (A). Data shown were independent points and mean; unpaired t-test; n = 6; ***p < 0.001. C–H Quantitative analysis of behavioral tests. Neurological deficits were evaluated by the mNSS test (C), foot fault assays (D), adhesive-removal test (E), rotarod assays (F), cylinder test (G), and corner test (H). Data shown were independent points and mean; two-way ANOVA; n = 6; **p < 0.01; ***p < 0.001. I–M Reduced MIF protein translocation to the nuclei of cortical neurons in MIF K78Q mice after ischemia. Ischemia was induced in WT and MIF K78Q mice. Brain sections were stained with anti-MIF antibody (I), anti-NeuN, and anti-γH2AX antibodies (K). J Quantification analysis of MIF intensity in nucleus/cytosol shown in (I). Data shown were mean ± SEM; unpaired t-test; n = 13 cells from three mice; ***p < 0.001. L, M Quantification analysis of NeuN-positive cells (L) and γH2AX-positive cells (M) shown in (K). Data shown were independent points and mean; unpaired t-test; n = 6 sections from three mice; ***p < 0.001.