Fig. 7: Inhibition of autophagy by 3-MA reduced the activity of proguanil in BC cells. | Cell Death & Disease

Fig. 7: Inhibition of autophagy by 3-MA reduced the activity of proguanil in BC cells.

From: Inhibitory role of proguanil on the growth of bladder cancer via enhancing EGFR degradation and inhibiting its downstream signaling pathway to induce autophagy

Fig. 7

A T24, J82 were plated in 6-well plates (3.0 × 105 /well). When the cell density reaches 70–80%, cells were treated with 3-MA (5 mM) and proguanil for 12 h. Western blotting was used to detect the protein expression of LC3 and Beclin-1. B T24, J82 were plated on glass disks in 12-well plates (2.0 × 104/well), After 24 h, cells were treated with 3-MA (5 mM) and proguanil, immunofluorescence was used to detect the protein expression of LC3. C Cells were plated in 96-well plates (6.0 × 103/well), After 12 h, cells were treated with 3-MA (5 mM) and proguanil, the inhibitory effects of proguanil and 3-MA on proliferation were detected by MTT. D Cells were plated in 24-well plates (2.0 × 103/well), After 12 h, cells were treated with 3-MA (5 mM) and proguanil, the he inhibitory effects of proguanil and 3-MA on proliferation were detected by colony formation assay. E The inhibitory effects of proguanil and 3-MA on migration were detected by transwell as described in “Materials and methods”. Data are representative of three independent experiments. Error bars represent means ± SD from triplicate experiments. Vehicle control means the concentration of DMSO lower than 0.3% (*P < 0.05, **P < 0.01, ***P < 0.001, ns not significant).

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