Fig. 3: RNAi of lola results in smaller ovaries and fewer ovarioles.

A Phenotypes of ovaries from the c587 > w1118 and c587 > lola12574 lines. Ovaries with normal size and number of ovarioles (a, a’) from the c587 > w1118 control line (n = 25), and ovaries with either one (b, c) or both (d) in smaller size and fewer ovarioles (c’) or lacking ovarioles (b’, d’) from the c587 > lola12574 RNAi line (n = 58). B Phenotypes of ovaries from the Tj > w1118, Tj > lola12574 and Tj > lola12052R-1 lines. Ovaries with normal size and number of ovarioles (e, e’) from the Tj > w1118 control, ovaries with either one (f) or both (g) in smaller size from the Tj > lola12574 RNAi line, and ovaries with one in smaller size (h) from the Tj > lola12052R-1 RNAi line. a’-h’ Higher magnifications of the ovary regions from the corresponding ovaries (a–h). c”, d” Higher magnifications of the ovary regions from the corresponding ovaries (c’, d’) showing DNA fragmentations. Scale bar: 200 µm in a–h, and 100 µm in a’–h’.