Fig. 4: RNAi of lola affects TF formation during WPP.

A Ovaries from the control c587 > w1118 line (a) and c587 > lola12574 RNAi line (b, c) at the WPP stage were immunostained with En antibody to mark TFCs (a’-c’, red), and nuclei were stained with DAPI (a”–c”, blue). In a control ovary (a–a”), TFs were well organized, while in c587 > lola12574 RNAi ovaries, either some TFCs were arranged randomly (b–b”) or no TFs were formed (c–c”). B The mean number of TFs per ovary in c587 > lola12574 RNAi ovaries is significantly lower than that of the c587 > w1118 control ovaries (n = 20). Significant difference was determined by the student’s t-test and indicated by ***p < 0.001. Scale bar: 30 µm.