Fig. 7: Lola regulates ovarian morphogenesis by suppressing Roc1b expression.
A Phenotypes of ovaries from the c587 > w1118, c587 > UAS-Roc1b34048, c587 > lola12574;Roc1b31067, c587 > lola12574;GFP and c587 > Roc1b31067 lines. a Ovaries with normal size and number of ovarioles from the c587 > w1118 control. b, c Overexpression of Roc1b resulted in one smaller ovary with fewer ovarioles (b) or a pair of smaller ovaries with fused egg chambers (c), which mimic the phenotypes of lola12574 RNAi. d Knockdown of Roc1b in the c587 > lola12574 RNAi line rescued defects in ovarian morphogenesis. e, f Knockdown of GFP in the c587 > lola12574 RNAi line resulted in one smaller ovary with fewer ovarioles (e) or a pair of smaller ovaries with fused egg chambers (f). g Ovaries with normal size and number of ovarioles from the c587 > Roc1b31067. c’ Higher magnification of the ovary region from c. B Knockdown expression of Roc1b in flies (c587 > Roc1b31067) did not have an effect on female fertility, while knockdown expression of Roc1b in the c587 > lola12574 RNAi line increased female fertility. C Expression of Roc1b in the ovaries of the c587 > Roc1b31067 and c587 > w1118 control lines. Significant difference was determined by the student’s t-test and indicated by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns for non-significant. Scale bar: 100 µm in a–g, 300 µm in c’.
