Fig. 3: Transcriptomic features of monocyte-derived cells under SULT2B1b perturbation. | Cell Death & Disease

Fig. 3: Transcriptomic features of monocyte-derived cells under SULT2B1b perturbation.

From: Harnessing the reverse cholesterol transport pathway to favor differentiation of monocyte-derived APCs and antitumor responses

Fig. 3

A Principal Component Analysis (PCA) of the transcriptomes of mono-DCs and Ly6Clow cells isolated from LLC-Mock and LLC-SULT2B1b tumors, integrated with 4 datasets present in the GEO (Gene Expression Omnibus) Database. PCA based on the expression values of 10080 genes detected in all the five experimental datasets. The first two principal components are shown (PC1 accounted for 23.4 % and PC2 for 18.3% of the variance among samples). B Uniform Manifold Approximation and Projection analysis (UMAP) of the transcriptomes as reported in (A). The figure shows bidimensional UMAP representation of the integrated dataset. Four different clusters have been identified using graph-based techniques. C Hierarchical clustering analysis. Hierarchical clustering of the expression values of 10080 genes detected with average linkage was performed on the specified subsets to assess their transcriptional relatedness. Spearman correlation-based distance was used.

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