Fig. 5: F4/80+TAM greatly promoted tumor vascularization in vitro and in vivo. | Cell Death & Disease

Fig. 5: F4/80+TAM greatly promoted tumor vascularization in vitro and in vivo.

From: Dynamic differentiation of F4/80+ tumor-associated macrophage and its role in tumor vascularization in a syngeneic mouse model of colorectal liver metastasis

Fig. 5

A The immune infiltration analysis by mMCP-counter with the bulk RNA-seq data of F4/80+ cells and CD11b+ cells extracted from CRLM showed mainly macrophages. GSEA analysis showed F4/80+TAM was greatly linked to vascularization. B Co-culture of ex vivo F4/80+TAM from CRLM with the endothelial cells (SVEC4–10, S) under TCM (Mc38 cell conditioned medium) greatly increased the expression of angiogenesis markers in SVEC4–10 cells in vitro by RT-PCR. C F4/80+TAM and CD11b+TAM showed distinct angiogenesis profiles from those in the liver and varied CRLM at the transcriptomic level by bioinformatics analysis. *p < 0.05; **p < 0.01; ***p < 0.001.

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