Fig. 6: Y658 phosphorylation of VE-cadherin is essential in the ability to form VM by stabilization of β-catenin and TCF-4 gene transcription.
From: VE-Cadherin modulates β-catenin/TCF-4 to enhance Vasculogenic Mimicry
A–C Western blot assay, VE-Cad K.O, VE-Cad K.O WT VE-Cad, VE-Cad K.O Y658F construct (1µgr during 48 h) in MUM 2B showed that VE-cadherin/Y658 is essential for β-catenin stabilization. D Immunoprecipitation after cytosol-nucleus fractionation of VE-Cadherin in MUM 2B cells, VE-Cad K.O, VE-Cad K.O WT VE-Cad, VE-Cad K.O Y658F construct (1 µgr during 48 h), input was used to protein expression controls, α-tubulin cytosol control or PARP-1 nucleus control. E Tumor growth progression graphic representation on different days, the annotations of the tumor were every two days, being the day 4 post-injection of MUM 2B cells, VE-Cad K.O, VE-Cad K.O WT VE-Cad, VE-Cad K.O Y658F construct (1 µgr during 48 h) before the beginning the injection of the cells (N = 6 per group). Results are represented with asterisks that denote significance in an unpaired t-test (p < 0.05, p < 0.01, p < 0.001), and error bars denote SD.
