Fig. 4: RNF157-AS1 served as a modular scaffold to epigenetically suppress DIRAS3 and ULK1 expression. | Cell Death & Disease

Fig. 4: RNF157-AS1 served as a modular scaffold to epigenetically suppress DIRAS3 and ULK1 expression.

From: Differential effects of the LncRNA RNF157-AS1 on epithelial ovarian cancer cells through suppression of DIRAS3- and ULK1-mediated autophagy

Fig. 4

A Differentially expressed mRNAs after RNF157-AS1 knockdown with hierarchical clustering. B Differentially expressed mRNAs shown on a volcano plot. C and D RNF157-AS1 expression had a negative correlation with DIRAS3 expression (R = −0.39, P = 1.2*10−19) and ULK1 expression (R = −0.3, P = 8.4*10−12). E–G The mRNA and protein expression level of DIRAS3 after RNF157-AS1 knockdown or overexpression. Full-length blots are presented in Supplementary Figs. S5 and S6. H–J The mRNA and protein expression level of ULK1 after RNF157-AS1 knockdown or overexpression. Full-length blots are presented in Supplementary Figs. S5 and S6. K and L ChIP-qPCR assay demonstrated that inhibiting RNF157-AS1 suppressed the binding of EZH2 to the promoter of the DIRAS3 genes and that RNF157-AS1 overexpression enhanced the binding of EZH2 to the promoter of the DIRAS3. M and N The ChIP assay demonstrated that diminishing of RNF157-AS1 decreased the binding of HMGA1 to the promoter of ULK1 genes and RNF157-AS1 overexpression enhanced the binding of HMGA1 to the promoter of ULK1. Data were obtained from at least three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 (t-test).

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