Fig. 5: RNF157-AS1 inhibited autophagy in EOC cells. | Cell Death & Disease

Fig. 5: RNF157-AS1 inhibited autophagy in EOC cells.

From: Differential effects of the LncRNA RNF157-AS1 on epithelial ovarian cancer cells through suppression of DIRAS3- and ULK1-mediated autophagy

Fig. 5

A MDC/PI dual staining in SKOV3 cells. The bar represents 100 μm. B Quantitative analysis of the IOD indicated RNF157-AS1 knockdown increased the MDC staining and RNF157-AS1 overexpression decreased MDC staining, but there was no significant difference in PI staining. C Autophagy/cytotoxicity dual staining in A2780 cells. The bar represents 100 μM. D Quantitative analysis of the MDC or PI staining indicated RNF157-AS1 knockdown increased autophagy level and RNF157-AS1 overexpression decreased autophagy level, but it had no effect on apoptosis. E LC3 staining in SKOV3 cells after RNF157-AS1 knockdown or overexpression. The bar represents 10 μm. F Quantitative analysis of the relative number of LC3 puncta (LC3 II) in every cell. G The LC3 II and LC3 I expression was detected by western blot in SKOV3 cells and A2780 cells after RNF157-AS1 knockdown or overexpression. Full-length blots are presented in Supplementary Fig. S7. H Quantitative analysis of the ratio of LC3 II/LC3 I in SKOV3 cells and A2780 cells after RNF157-AS1 knockdown or overexpression (1: si-NC; 2: si-RNF157-AS1; 3: pcDNA3.1; 4: pcDNA3.1-RNF157-AS1). I Western blot analysis of the protein from A2780 cells transfected with RNF157-AS1 interference or overexpression plasmid and then treated with 3-MA (100 μM, M9281, Sigma) or CQ (100 μM, C6625, Sigma) for 6 h. Full-length blots are presented in Supplementary Fig. S8. J Quantitative analysis of the ratio of LC3 II/LC3 I/β-actin (1: si-NC; 2: si-RNF157-AS1; 3: pcDNA3.1; 4: pcDNA3.1-RNF157-AS1). Data were obtained from at least three independent experiments. *P < 0.05; **P < 0.01 (t-test).

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