Fig. 8: RNF157-AS1 exerts differential effects on EOC in different environments by suppressing autophagy in vivo.

A sh-NC and sh-RNF157-AS1 were stably transfected into A2780 cells and injected in the nude mice. B pLV-NC and pLV-RNF157-AS1 were stably transfected into A2780 cells and injected in the nude mice. C and D The tumor volumes and the tumor weights of sh-NC and sh-RNF157-AS1 were calculated. The bars indicated SEM. E and F The tumor volumes and the tumor weights of pLV-NC and pLV-RNF157-AS1 were calculated. The bars indicated SEM. G and H IHC staining of DIRAS3 and ULK1 in the tumor with stably transfecting sh-NC, sh-RNF157-AS1, pLV-NC, and pLV-RNF157-AS1. I After being injected with A2780 cells with stably transfecting sh-NC and sh-RNF157-AS1 for 2 weeks, the mice were treated with DDP twice a week. J pLV-NC and pLV-RNF157-AS1 were stably transfected into A2780 cells and injected in the nude mice for 2 weeks, the mice were treated with DDP twice a week. K and L The tumor volumes and the tumor weights of sh-NC and sh-RNF157-AS1 were calculated after treatment with DDP. The bars indicated SEM. M and N The tumor volumes and the tumor weights of pLV-NC and pLV-RNF157-AS1 were calculated after treatment with DDP. The bars indicated SEM. O and P IHC staining of DIRAS3 and ULK1 in the tumor with stably transfecting sh-NC, sh-RNF157-AS1, pLV-NC, and pLV-RNF157-AS1 after treated with DDP. Q Model explaining the mechanism of RNF157-AS1 in EOC cells in different environments. Data were obtained from at least three independent experiments. *P < 0.05; **P < 0.01 (t-test).