Fig. 1: DAPL1 deficiency promotes the EMT of RPE cells. | Cell Death & Disease

Fig. 1: DAPL1 deficiency promotes the EMT of RPE cells.

From: DAPL1 prevents epithelial–mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy

Fig. 1

A Real-time PCR showing the expression level of DAPL1 in proliferative vitreoretinopathy (PVR) membranes and primary human RPE cells. B Western blotting showing the protein expression levels of DAPL1, ZO-1, E-cadherin, ZEB1, N-cadherin, vimentin, and SNAIL in 8-week-old WT and Dapl1 − /− RPE cells. C The bar graph shows quantification of the bands based on the results in (B). D, E Immunostaining images of ZO-1 expression in RPE flat mounts of 2-week-old or 8-week-old WT and Dapl1−/− mice. Scale bar: 50 μm. F Histological images of H&E staining of the retinal structure showing that 8-week-old WT and Dapl1−/− mice were subretinally injected with sodium hyaluronate to induce retinal detachment for 10 days Scale bar: 100 μm. (G, H) Immunohistochemistry images showing the expression of OTX2 (white arrows in G) and α-SMA (white arrows in H) on the surfaces of the detached retinas. Scale bar: 50 μm. N = 6, ** or * indicates P < 0.01 or *P < 0.05, respectively.

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