Fig. 4: DAPL1 inhibits PVR progression in a rabbit model. | Cell Death & Disease

Fig. 4: DAPL1 inhibits PVR progression in a rabbit model.

From: DAPL1 prevents epithelial–mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy

Fig. 4

ARPE-19+EGFP or ARPE-19 + DAPL1 cells were injected into the vitreous body of pigmented rabbits to induce experimental PVR. A Representative images showing fundus photographs captured on day 38 in each group. Note that severe retinal detachment with retinal folds was observed in ARPE-19+EGFP-injected eyes but not in ARPE-19 + DAPL1-injected eyes. B Optical coherence tomography (OCT) scanning showing that severe retinal detachment could be observed in the ARPE-19+EGFP-injected eyes but not in the ARPE-19 + DAPL1-injected eyes after 38 days. C Representative histological images of H&E staining of the rabbit eye in each group were obtained and analyzed. Note that retinal folds (black triangles), retinal detachment, and the formation of the epiretinal and subretinal membranes (red stars) were observed in the ARPE-19+EGFP-injected eyes but not in the ARPE-19 + DAPL1-injected eyes. D Immunohistochemistry images showing the detection of α-SMA expression around the retinas in the rabbits; positive signals were observed in the epiretinal and subretinal membranes in ARPE-19+EGFP-injected eyes (yellow arrows). E The severity of PVR in each group was graded according to Fastenberg’s score at the indicated times. N = 6, Scale bar: 50 μm.

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