Fig. 5: DAPL1 inhibits the EMT of RPE cells by regulating P21. | Cell Death & Disease

Fig. 5: DAPL1 inhibits the EMT of RPE cells by regulating P21.

From: DAPL1 prevents epithelial–mesenchymal transition in the retinal pigment epithelium and experimental proliferative vitreoretinopathy

Fig. 5

A, B Western blots showing the expression of DAPL1 and P21 in ARPE-19 + DAPL1 cells and quantification (B). C, D At 2 weeks after infection with AAV9 or AAV9-DAPL1, western blots show the expression of DAPL1 and P21 in Dapl1 − /− mouse RPE cells and quantification (D). E Real-time PCR showing the knockdown efficiency of P21 in ARPE-19 + DAPL1 cells. F, G Western blots showing the expression of P21, ZO-1, E-cadherin, N-cadherin, Vimentin, and SNAIL in ARPE-19 + DAPL1 cells after the knockdown of P21 and quantification. H Immunostaining images for ZO-1, vimentin, and SNAIL in ARPE-19 + DAPL1 cells after the knockdown of P21. N = 3, ** or * indicates P < 0.01 or *P < 0.05, respectively.

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