Fig. 1: GMDS-AS1 indicates poor clinical outcomes in CRC.
From: LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axis
A Validation of GMDS-AS1 expression induced by IL-6 (10 ng/ml) through quantitative real-time PCR. B GMDS-AS1 RNA levels in 50 pairs of CRC tissues and adjacent normal tissues were detected by qPCR. ***p < 0.001. C Relative GMDS-AS1 RNA expression in 478 CRC patients in the TCGA COAD dataset cohort. High GMDS-AS1 positively correlated with later TNM stages. D Kaplan–Meier analyses showing the correlation between GMDS-AS1 RNA levels and overall survival in 268 TCGA COAD patients. The CRC patients were stratified on the basis of the median GMDS-AS1 RNA level (log-rank test). C, D The figure was generated with GEPIA2. E Kaplan–Meier analyses of the correlation between GMDS-AS1 RNA levels and relapse-free survival in 226 CRC patients in the Tumor Colon-Sieber-290-MAS5.0-u133p2 cohort (GSE14333). Patients were stratified on the basis of the upper quartile GMDS-AS1 value. F The expression levels of GMDS-AS1 were measured in CRC plasma samples from 97 CRC patients and 91 patients with gastrointestinal inflammation serving as control individuals by qPCR. The Mann–Whitney U test was performed to determine statistical significance. G Receiver-operating characteristic (ROC) curves and the corresponding area under the curve (AUC) values for the plasma GMDS-AS1 data presented in F.
