Fig. 3: STOML2 repressed PINK1-induced mitophagy in pancreatic cancer cells. | Cell Death & Disease

Fig. 3: STOML2 repressed PINK1-induced mitophagy in pancreatic cancer cells.

From: STOML2 restricts mitophagy and increases chemosensitivity in pancreatic cancer through stabilizing PARL-induced PINK1 degradation

Fig. 3

A–D Immunoblot showing the protein levels of PINK1, STOML2 and Vinculin in PANC1 and BxPC3 cells after downregulating or upregulating STOML2, which were treated with CCCP (10 μM) or vehicle in cell medium for 24 h. E–H Immunoblot showing the protein levels of LC3BI, LC3BII, STOML2, and Vinculin in PANC1 and BxPC3 cells after downregulating or upregulating STOML2, which were treated with CQ (10 μM) and CCCP (10 μM) in cell medium for 24 h. The ratios of LC3BII/LC3BI were calculated by the OD value of the corresponding blot band determined by ImageJ. I–J Fluorescent staining showed the colocalization between lysosomes and mitophagy (mitochondria) in PANC1 and BxPC3 cells after downregulating or upregulating STOML2. The quantitative results were calculated by CellProfiler V4.2.1. Scale bar equals 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001.

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