Fig. 6: USP1 regulates TAZ protein stability by inhibiting K11-linked polyubiquitination of TAZ.

A Western blot analysis of polyubiquitinated TAZ was performed after coimmunoprecipitation (Co-IP) in HLF cells treated as indicated. B, C Western blot analysis of polyubiquitinated TAZ was performed after coimmunoprecipitation in HEK293 cells treated as indicated. D, E Western blot analysis of K11 linkage-specific polyubiquitinated TAZ was performed after coimmunoprecipitation in HLF or HEK293 cells treated as indicated. F Western blot analysis of K11R linkage-specific polyubiquitinated TAZ was performed after coimmunoprecipitation in HEK293 cells treated as indicated. G The hypothetical mechanistic model of USP1-regulated Hippo signaling in HCC progression. The USP1 associates with TAZ and decreases TAZ protein degradation via K11-linked polyubiquitination of TAZ, which increases Hippo/TAZ axis activation and enhances malignant progression in HCC cells. All the results are representative of three independent experiments.