Fig. 1: CYP1B1 is involved in ferroptosis.
From: CYP1B1 inhibits ferroptosis and induces anti-PD-1 resistance by degrading ACSL4 in colorectal cancer
A Western blot analysis of RKO, HCT116 and HT29 cells expressing empty vector (EV) or Flag-CYP1B1. B Western blot analysis of RKO, HCT116, and HT29 cells expressing control (ctrl) shRNA or CYP1B1 shRNA. C, D Viability curves of RKO, HCT116 and HT29 cells with CYP1B1 overexpression or knockdown treated with the indicated concentrations of RSL3. E–H Lipid peroxidation levels were tested using C11-BODIPY in CYP1B1 overexpression (E, F) or knockdown (G, H) RKO cells. The data shown represents the mean ± SD; n = 3. p value was determined by Student’s two-sided t test. **p < 0.01, ***p < 0.001.
