Fig. 5: miR-182 enhances IL-8 expression in NSCLC cells through targeting KLHL21. | Cell Death & Disease

Fig. 5: miR-182 enhances IL-8 expression in NSCLC cells through targeting KLHL21.

From: A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer

Fig. 5

A KLHL21 was predicted to be a target of miR-182. Top, predicted miR-182 regulatory elements (seed sequences in upper case) in KLHL21 3′UTR. Bottom, sequences of the wild-type (pRL-TK-KLHL21 3′UTR) and the mutated (pRL-TK-KLHL21 3′UTR mut) KLHL21 3′UTR luciferase reporters. B Luciferase reporter assay for the KLHL21 3′UTR reporter in 293T cells. C Western blot and qPCR analyses of KLHL21 protein (left) or mRNA levels (right) in miR-182-overexpressing A549 cells. D Western blot and qPCR analyses of KLHL21 protein (left) or mRNA levels (right) in anti-miR-182–transfected A549 cells. E, F qPCR (E) and ELISA (F) analyses of the effect of ectopic expression of KLHL21 on IL-8 expression and secretion in miR-182-overexpressing A549 cells. The average values ± SEM of three separate experiments are plotted. *P < 0.05; **P < 0.01; n.s., not significant.

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