Fig. 6: IL-8 activates osteoclastogenesis via STAT3 signaling. | Cell Death & Disease

Fig. 6: IL-8 activates osteoclastogenesis via STAT3 signaling.

From: A novel microRNA-182/Interleukin-8 regulatory axis controls osteolytic bone metastasis of lung cancer

Fig. 6

A Western blotting of the effects of miR-182-CM or IL-8 on STAT3 phosphorylation and NFATc1 expression in RAW264.7 cells. Quantification of blotting intensity is shown in parentheses (the one in control cells is set as 100% after normalization with β-actin). B, C Western blotting of the effects of JAK/STAT3 signaling inhibitor AG-490 and RANKL decoy receptor Osteoprotegerin (OPG) on STAT3 phosphorylation and NFATc1 expression in miR-182-CM (B) or IL-8 (C) -treated RAW264.7 cells, with quantification of blotting intensity shown in underneath (the one in control cells is set as 100% after normalization with β-actin). D TRAP staining analysis of the effect of AG-490 treatment on osteoclast differentiation of miR-182-CM or IL-8-treated RAW264.7 cells. Arrowheads indicate mature, multi-nucleated osteoclasts. Scale bars, 50 μm. The average values ± SEM of three separate experiments are plotted. **P < 0.01.

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