Fig. 2: The characteristics of MA9-IL-34 cells.

A–D The mice were transplanted with MA9 or MA9-IL-34 cells on day 0 and sacrificed on day 16. A Mice were sacrificed 16 h after intraperitoneal injection of 200 μg BrdU. Then assays were performed following standard protocols. The representative flow cytometric results are shown (upper), and the percentages of G0/G1-, S- and G2/M-phase AML cells are plotted (lower). B Annexin V and PI staining was performed following standard protocols. The representative flow cytometric results are shown (upper), and the percentage of apoptotic AML cells is plotted (lower). C–D Primary (C) and secondary (D) colony forming assays were performed when 500 AML cells in M3434 medium were seeded each well into 24-well plates and cultured for 7d. The representative results are shown (upper), and colony numbers are plotted (lower). E, F Different numbers of sorted AML cells (5 × 10⁴, 5 × 10³, 5 × 10² for each group) were transplanted into recipient mice (n = 9 to 10 for each group). The survival of mice is shown in Kaplan–Meier curves (E). The frequency of LSCs was calculated using ELDA software (F). G AML cells were stained with c-Kit. The representative flow cytometric results are shown (left), and the percentage of c-Kit⁺ cells is plotted (right). H Equal numbers of sorted MA9-c-Kit^-, MA9-c-Kit⁺ and MA9-IL-34 cells were transplanted into recipient mice. The survival of mice is shown in Kaplan–Meier curves (n = 8 for each group). Data are presented as mean ± S.E.M. Unpaired Student’s t test, one-way ANOVA tests and Kaplan–Meier estimates were used. *p < 0.05, **p < 0.01, ***p < 0.001.