Fig. 2: Mdm2 haploinsufficiency in MSCs promotes thrombocytopenia after IR induced cellular stress. | Cell Death & Disease

Fig. 2: Mdm2 haploinsufficiency in MSCs promotes thrombocytopenia after IR induced cellular stress.

From: Mdm2/p53 levels in bone marrow mesenchymal stromal cells are essential for maintaining the hematopoietic niche in response to DNA damage

Fig. 2

A Schematic view of experiment design. B Bar graph showing platelet counts in the indicated mice 10 days after irradiation. Data points represent the platelet count in each mouse, (mean ± SD); ****p < 0.0001, ***p < 0.001, **p < 0.01, Student’s t test. C Representative microscopic view of hematoxylin and eosin (H&E) staining of BM sections isolated from the indicated mice 2 weeks after IR. D High magnification microscopic view of H&E staining of BM sections in indicated mice after IR. The black arrows point to megakaryocytes. Kaplan-Meier plot of the indicated mice after irradiation. E Quantification of the megakaryocytes from D. The Y-axis of the graph represents the number of megakaryocytes per square millimeter (mm2) of bone marrow section using a 20× objective and a standard area of 2.6 mm2. (control n = 10; Osx-Mdm2fl/+ n = 6; Vav-Mdm2fl/+ n = 6). **P < 0.01. G TUNEL staining of a BM sample derived from Osx-Cre;mTmG mouse after IR. The white arrows point to MSCs (GFP + ). H Immunostaining of p53 in Osx-Mdm2fl/+ mouse after irradiation.

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