Fig. 3: Reduced HK2 expression in HIF-1α CTAD−/− mice is associated with hypoxia-induced kidney injury. | Cell Death & Disease

Fig. 3: Reduced HK2 expression in HIF-1α CTAD−/− mice is associated with hypoxia-induced kidney injury.

From: Activation of HIF-1α C-terminal transactivation ___domain protects against hypoxia-induced kidney injury through hexokinase 2-mediated mitophagy

Fig. 3

a Hierarchical clustering shows distinct mRNA expression profiling between the HIF-1α CTAD KO and WT kidneys with I/RI. The red and green shades indicate the expression above and below the relative expression, respectively, across all samples. b Volcano plot showing differentially expressed genes. The up‐regulated genes (red), and the down‐regulated genes (green) with a fold change <0.6, and with p < 0.05. c The gene ontology category analysis of differentially expressed genes. d The pathway enrichment analysis of differentially expressed genes. e, f HK2 mRNA expression in the kidney with I/RI (e) or UUO (f) detected by qRT-PCR. The relative levels were normalized to β-actin (n = 6). g, h Western blotting analysis of HK2 expression in the kidney with I/RI (g) or UUO (h) (n = 6). i, j Immunohistochemical analysis of HK2 expression in the kidney with I/RI (i) or UUO (j) (n = 6). Scale bars, 50 µm for I/RI; 100 µm for UUO. Data are shown as mean ± SEM of 6 mice. **p-value < 0.01 versus the WT mice (t-test).

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