Fig. 5: UHRF1 silencing downregulated GLI1 through CEBPA. | Cell Death & Disease

Fig. 5: UHRF1 silencing downregulated GLI1 through CEBPA.

From: UHRF1 inhibition epigenetically reprograms cancer stem cells to suppress the tumorigenic phenotype of hepatocellular carcinoma

Fig. 5

A Immunofluorescence analysis of GLI1 (red) and UHRF1 (green) expression in UHRF1 knockdown and control CRL-8024 cells. The nuclei were stained with DAPI (blue). Scale bar: 100 μm. B Protein levels of UHRF1 and GLI1 in liver tissues from Uhrf1HKO mice and corresponding control mice at different time points in the DEN/CCl4 induced HCC model. C Venn diagram among three datasets in CRL-8024 cells. The relative expression of overlapping genes is represented in a heatmap. D The relative mRNA expression of genes in CRL-8024 cells after CEBPA or GATA3 silencing. E The GLI1 promoter was cloned into the pGL3-basic vector and cotransfected with siCEBPA or scramble siRNA for dual-luciferase assays (low/high: low/high concentration of siRNA) (n = 3). Mean ± SD. P values were determined using unpaired Student’s t test. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗∗P < 0.0001. Ns not significant.

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